|
KMID : 0043319950180020113
|
|
Archives of Pharmacal Research
1995 Volume.18 No. 2 p.113 ~ p.117
|
|
|
Preparation of δ-Opioid Receptor-Sepcific Antibodies Using Molecular Cloned Genes
|
|
Kim Ae-Young
Lee Shee-Yong
Kim Kyeon-Min
|
|
|
Abstract
|
|
|
|
We re-cloned mouse receptor from NG108-15 cells using RT-PCR, and confirmed it by restriction analysis and by sequencing the beginning and end part of the amplified DNA. When transiently expressed in COS-7 cells, cloned receptor showed saturable and specific binding to naloxone with very similar binding parameters to originally reported ones. To make antibodies specific for the receptor, the carboxy tail of the receptor, which is unique to the receptor compared with other opioid receptors, was expressed in bacteria as a ufsion proteinwith glutathione S-transferase. Purified fusion protein selective for receptor when tested by western blotting using membrane proteins prepared from transfected COS-7 cells. Cloned receptor andl antibodies specific for receptor are going to be valuable tools for studying pharmacological actions of the receptor and morphine dependence.
|
|
|
KEYWORD
|
|
|
δ-Opioid receptor, Antibodies Cloning, Binding study
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|